Snapshot: Light relief

SnapShot: Light Microscopy

Total internal reflection fluorescence (TIRF) microscopy fluorescence— optical sectioning limiting the excitation volume to the close vicinity of the interface by sending the excitation light at an incident angle higher than the critical angle; usually implemented using a high numerical aperture (NA) objective. high signal-to-background for nearsurface structures monitoring plasma membrane proc...

White-light Sagnac interferometer for snapshot multispectral imaging.

The theoretical and experimental demonstration of a multispectral Sagnac interferometer (MSI) is presented. The MSI was created by including two multiple-order blazed diffraction gratings in both arms of a standard polarization Sagnac interferometer (PSI). By introducing these high-order diffractive structures, unique spectral passbands can be amplitude modulated onto coincident carrier frequen...

Light induced conch-shaped relief in an azo-polymer film

We have discovered that a novel chiral structured surface relief (termed 'conch'-shaped surface relief) with a height of over 1 μm can be formed in an azo-polymer film merely by employing circularly polarized optical vortex irradiation with a total angular momentum of j = ±2. The temporal evolution of the conch-shaped surface relief in the azo-polymer film was also observed. The results provide...

SnapShot: melanoma.

SnapShot: Macroautophagy

(Middle) Cellular Events in Macroautophagy Macroautophagy (hereafter referred to as autophagy) is a catabolic process in which portions of the cytoplasm are sequestered within double-or multimembraned vesicles termed autophagosomes and then delivered to lysosomes for bulk degradation. The initial phases of macroautophagy consist of the formation of a phago-phore, which is also called an isolati...